Ki-67 (D3B5) Rabbit mAb 9129S from Cell Signaling Technology

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Theranostics
January 01 2020

Fig 1: Me6 promoted intestinal stem cell proliferation and crypt regeneration post irradiation. (A) Representative HE-stained sections and the quantification of the villus and crypt length of small intestine at 24 and 96 h after 14 Gy WBI and different treatments (*p < 0.05; Scale bar = 100 µm). (B-C) Proliferation of the crypts of small intestine at 24 and 96 h after 14 Gy WBI and different treatments, as assessed by Ki67 (B) and CyclinD1 (C) staining, respectively (*p < 0.05, **p < 0.01; Scale bar = 100 µm). Three mice were used in each group. (D-E) Heat map showing the proliferation (D) and ISC-related genes (E) that are upregulated in the intestinal crypts from Me6-treated mice at 96 h after 14 Gy WBI. Microarray was performed using mRNA isolated from the intestinal crypts. (F) qPCR detection for ISC-related gene expression in the intestinal crypts at 96 h after 14 Gy WBI and different treatments (**p < 0.01). (G) Representative Lgr5-stained intestinal sections and the Lgr5+ cell numbers in the crypts at 24 and 96 h after 14 Gy WBI and different treatments (**p < 0.01; Scale bar = 100 µm). Three mice were used in each group. (H) Representative images of in situ hybridization for Lgr5 mRNA in the intestinal crypts at 96 h after 14 Gy WBI and different treatments. (I) Representative Sox9-stained sections and the quantification of Sox9+ cells in the crypts of the small intestine after 14 Gy WBI and different treatments (**p < 0.01; Scale bar = 100 µm). Three mice were used in each group. (J) The quantification of regenerated crypts at 96 h after 14 Gy WBI and different treatments, as indicated by BrdU incorporation for 2 h (**p < 0.01; Scale bar = 100 µm). Three mice were used in each group. (K) The number of crypt fission per filed on murine jejunum sections. Six mice were used in each group (**p < 0.01).
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Journal of the American Heart Association
July 14 2017

Fig 2: GLP‐1R stimulation redressed the activated CD150+ cell proliferation in response to stress. A, Gating for the LSK cell population isolated from BM cells of stress‐alone (S) and stressed‐exenatide treatment (S‐Ana) group mice (upper panels) and subanalyzed Lin−c‐KithighSca‐1high CD48low CD150high HSC (lower panels). B, BM LSK and HSC numbers next to gates represent population frequencies (%) of 2 experimental groups after 4 weeks of stress (n=6–8, Student t test). C, Quantitative data for the numbers of PCNA +/CD150+ cells in the BM niches. D, FACS analysis shows the numbers of Ki67/+ CD150+ cells in the BM. E, Representative histogram of DNA content during the cell cycle (left) and the distribution of S/M/G2 cells expressed as a percentage of total BM cells (right) (n=6, Mann–Whitney U test). F, Immunoreactive staining of BM niches for Adrβ3. Bar graphs: The percentage of positive area (mm2) (n=6 control, n=5 S‐Exe, Student t test). F, Representative Western bands for brain GLP‐1R protein expression. H, Representative gelatin zymography images for pro‐MMP9 and active‐form MMP9 activities in the BM sca‐1+ cells of the control and S‐Exe mice. Data are mean±SEM. *P<0.01 vs stress‐alone control mice. BM indicates bone marrow; FACS, fluorescence‐activated cell sorter; GLP‐1R, glucagon‐like peptide‐1 receptor; HSC, hematopoietic stem cells; LSK, lin−sca‐1+c‐Kit+; MMP9, matrix metalloproteinase 9; PCNA, proliferating cell nuclear antigen; S‐Ana, stressed anagliptin treatment; S‐Exe, stressed‐exenatide treatment.
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International Journal of Molecular Sciences
June 23 2021

Fig 3: Percentage of proliferating NEUROG3+ endocrine precursors is higher in the Neurod1CKO pancreas compared to the littermate controls. (A,B) Immunostaining for proliferating cell nuclear antigen Ki67 (red) in vibratome sections of the control and Neurod1CKO pancreas at E15.5 (arrows indicate NEUROG3+ proliferating cells). Nuclei are stained with Hoechst. (C) Quantification of proliferating NEUROG3+ cells per NEUROG3+ population (n = 3 embryos per genotype and 3–4 fields per section). Data are presented as mean ± SD, Unpaired t-test (* p = 0.03). Scale bar: 50 μm.
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Aging (Albany NY)
February 15 2021

Fig 4: LINC00485 regulates cell proliferation, migration, and invasion by sponging miR-581. (A, B) Cell viability of LINC00485 knockdown FHC cells with or without miR-581 antagomir treatment for 24 h and (B) LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was evaluated by the CCK-8 assay. (C) The percentage of Ki-67-positive cells in (C) LINC00485 knockdown FHC cells with or without miR-581 antagomir treatment for 24 h was tested by flow cytometry. (D) The percentage of Ki-67-positive cells in LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was evaluated by flow cytometry. (E, F) Colony forming capability of (E) LINC00485 silenced FHC cells with or without miR-581 antagomir treatment for 24 h and (F) LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was measured by the colony formation assay. (G, H) The migratory and invasive abilities of (G) LINC00485-silenced FHC cells with or without miR-581 antagomir treatment for 24 h and (H) LINC00485-overexpressed LoVo cells with or without miR-581 mimics for 24 h were detected by Transwell assays. Data was analyzed using the one-way ANOVA with LSD test. Bars were represented as S.D. *P<0.05. sh, short hairpin RNA targeting LINC00485; OE, overexpression.
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Redox biology
September 01 2020

Fig 5: RAD51 depletion reduces proliferation of ovarian cancer cells in vitro and impedes tumor growth in vivo. (A) RNAi efficiency of A2780, HEY and HO8910 cells were measured by Western blot analysis. α-Tubulin was used as a loading control (left). Band intensities were quantified by ImageJ. Colony-forming efficiency of A2780 and HO8910 cells after transfected scrambled siRNA (siScr) or siRNA targeting RAD51 (siRAD51) for two weeks (right). (B) Knockdown efficiency of RAD51 shRNA were evaluated after doxycycline treatment for 48 h by Western blot analysis (left). α-Tubulin was used as a loading control. Band intensities were quantified by ImageJ. Colony-forming efficiency of HO8910 cells stably transfected RAD51 shRNA after doxycycline treatment for two weeks (right). (C) EdU incorporation was performed after doxycycline treatment for 48 h in HO8910 cells. (D) Cell cycle distribution of A2780, HO8910 and HEY cells after transfected siScr or siRAD51 for 48 h. The cell cycle distribution was measured by flow cytometry. (E) Images of HO8910 shControl and HO8910 shRAD51 tumors of each treatment group. (F) Tumor weights at day 24. (G) Growth curves of tumors from transplanted HO8910 shControl and HO8910 shRAD51 cells in nude mice. Tumor volumes were measured every 3 days. (H) Representative images of immunohistochemistry staining using Ki-67 antibody in HO8910 tumors for each group. Scale bar: 50 μm. Data presented as mean ± S.D. are representative of three independent experiments. The statistical differences between the two groups were analyzed by two-sided unpaired Student's t-test (**p < 0.01, ***p < 0.001).

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Cell Signaling Technology for
Ki-67 (D3B5) Rabbit mAb

Cell Signaling Technology's Ki-67 (D3B5) Rabbit mAb is a Rabbit monoclonal antibody. The Ki-67 (D3B5) Rabbit mAb Antibody was generated using MKI67 as the antigen. It reacts with Homo Sapiens, Human, Mouse, and Rat. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, and Immunofluorescence.

Description

Ki-67 (D3B5) Rabbit mAb recognizes endogenous levels of total Ki-67 protein

Product Specs
ItemKi-67 (D3B5) Rabbit mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9129S
Quantity100 µl
ApplicationsIF-F, IF-IC, FC-FP
ReactivityH, M, R
ClonalityMonoclonal
TargetKI-67
HostRabbit
NCBI Full Gene Namemarker of proliferation Ki-67
NCBI Gene AliasesKIA, MIB-, MIB-1, PPP1R105
Research AreaCell Cycle / Checkpoint Control
IsotypeIgG
CloneD3B5
Molecular Weight359

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Cell Signaling Technology

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Phone: 978-867-2388
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Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Sustained release of vascular endothelial growth factor A and basic fibroblast growth factor from nanofiber membranes reduces oxygen/glucose deprivation-induced injury to neurovascular unitsNeural Regeneration ResearchApril 1, 2024Yifang Wu, Jun Sun, Qi Lin, Dapeng Wang, Jian Haifluorescence intensity. Primary antibodies were rabbit anti-Ki67 (1:400, CST, Danvers, MA, USA, Cat# 9129, RRID: AB_2687446), rabbit anti-NSE (1:100, Abcam, Cambridge, MA, USA, Cat# ab180943, RRID: AB_2934109),

(2) NRG1 Regulates Proliferation, Migration and Differentiation of Human Limbal Epithelial Stem CellsCurr Issues Mol BiolDecember 14, 2023Bofeng Wang, Huizhen Guo, Zhuo Han, Siqi Wu, Jiafeng Liu, Zesong Lin, Fengjiao An, Jin Zhu, Mingsen Lianti-ALDH3A1 (GeneTex, Irvine, CA, USA, #GTX30042), Anti-KI67 (Cell Signaling Technology, Danvers, MA, USA, #9129S) and Anti-TP63 (Cell Signaling Technology, Danvers, MA, USA, 67825S). 2.4. In Vitro CEC Differentiation When

(3) Circular RNA ZNF800 (hsa_circ_0082096) regulates cancer stem cell properties and tumor growth in colorectal cancerBMC CancerNovember 10, 2023Vimalan Rengganaten, Chiu-Jung Huang, Mong-Lien Wang, Yueh Chien, Ping-Hsing Tsai, Yuan-Tzu Lan, Hooi Tin Ong, Shih-Hwa Chiou, Kong Bung Choofor Lgr5 (ab219107, Abcam, UK), Sox9 (14–9765-82, eBioscience, Thermo Fisher Scientific) or Ki-67 (#9129, Cell Signalling Technology, MA, USA). Goat anti-mouse IgG secondary antibody conjugated with Alexa

(4) Serum starvation-based method of ovarian cancer cell dormancy induction and termination in vitroBiology Methods and ProtocolsNovember 2, 2023Szymon Rutecki, Agnieszka Leśniewska-Bocianowska, Klaudia Chmielewska, Julia Matuszewska, Eryk Naumowicz, Paweł Uruski, Artur Radziemski, Justyna Mikuła-Pietrasik, Andrzej Tykarski, Krzysztof Książekincubated with rabbit mAb against human Ki67 (D3B5, Cell Signaling Technology, Danvers, MA, cat. no. 9129 T), diluted to 1:400 in the blocking buffer at 4°C overnight. Afterward, the cells were incubated with

(5) Enabling Survival of Transplanted Neural Precursor Cells in the Ischemic BrainAdvanced ScienceNovember 1, 2023Zhifu Wang, Danyi Zheng, Ye Sing Tan, Qiang Yuan, Fang Yuan, Su‐Chun Zhang,Merck Millipore,MAB377,"https://www.merckmillipore.com/CN/zh/product/Anti‐NeuN‐Antibody‐clone‐A60,MM_NF‐MAB377" anti‐CCR5,Abcam,ab110103,https://www.abcam.com/ccr5‐antibody‐t218‐ab110103.html anti‐Ctip2,Abcam,ab18465,https://www.abcam.com/ctip2‐antibody‐25b6‐ab18465.html anti‐Brn2,Proteintech,14596‐1‐AP,https://www.ptgcn.com/products/POU3F2‐Antibody‐14596‐1‐AP.htm anti‐Foxp2,Abcam,ab16046,https://www.abcam.com/foxp2‐antibody‐ab16046.html anti‐Ki67,Cell Signaling Tech,9129S,https://www.cellsignal.com/products/primary‐antibodies/ki‐67‐d3b5‐rabbit‐mab/9129 anti‐Sox9,R&D Systems,AF3075,https://www.rndsystems.com/products/human‐sox9‐antibody_af3075 anti‐S100β,Abcam,ab52642,https://www.abcam.com/s100‐beta‐antibodyep1576y‐astrocyte‐marker‐ab52642.html anti‐CSPG,Abcam,ab11570,https://www.abcam.com/chondroitin‐sulfate‐antibody‐cs‐56‐ab11570.html anti‐Iba1,Wako

(6) Cancer-associated fibroblasts actively compress cancer cells and modulate mechanotransductionNature CommunicationsNovember 1, 2023Jorge Barbazan, Carlos Pérez-González, Manuel Gómez-González, Mathieu Dedenon, Sophie Richon, Ernest Latorre, Marco Serra, Pascale Mariani, Stéphanie Descroix, Pierre Sens, et al.antibody,–,1– 200,–,1–200,–,H0412,Sigma Aldrich Ki67,Anti-Ki67 rabbit monoclonal antibody,–,1–200,–,1–400,–,9129S,Cell Signaling EpCAM,"PE/Cy7 anti-human CD326, mouse IgG2b, κ",1–10,–,–,–,–,324221,Biolegend EpCAM,"APC

(7) Tumor Microenvironment Responsive CD8+ T Cells and Myeloid‐Derived Suppressor Cells to Trigger CD73 Inhibitor AB680‐Based Synergistic Therapy for Pancreatic CancerAdvanced ScienceNovember 1, 2023Qiangda Chen, Hanlin Yin, Junyi He, Yuqi Xie, Wenquan Wang, Huaxiang Xu, Lei Zhang, Chenye Shi, Jun Yu, Wenchuan Wu, et al.CXCR2 (Proteintech, 19538‐1‐AP), CXCL5 (R&D Systems, AF433), LY6G (Servicebio, GB11229), Ki‐67 (CST, 9129), and cleaved caspase 3 (CST, 9664), followed by biotinylated or fluorescent secondary antibodies. The

(8) Low-Density Lipoprotein Contributes to Endometrial Carcinoma Cell Proliferation, Migration, and Invasion by Activating the JAK–STAT Signaling PathwayAnalytical Cellular PathologyOctober 20, 2023Lifan Shen, Chen Zhang, Kaiying Cui, Xin Liang, Genhai Zhuprimary antibodies against caspase-3 (#9662, Cell Signaling Technology, Danvers, MA, USA) and Ki-67 (#9129, Cell Signaling Technology), and subsequently washed with PBS. The sections were then incubated with

(9) Transcription and chromatin regulation by TAF4b during cellular quiescence of developing prospermatogoniaFrontiers in Cell and Developmental BiologyOctober 12, 2023Megan A. Gura, Myles A. Bartholomew, Kimberly M. Abt, Soňa Relovská, Kimberly A. Seymour, Richard N. Freimanrat anti-TRA98 (1:100, abcam, ab82527), anti-KI67 rabbit mAb (1:100, Cell Signaling Technology D3B5, 9129S) and anti-PCNA (1:100, Cell Signaling Technology, 13110T). For immunofluorescence, the secondary antibodies

(10) MRTF-A gain-of-function in mice impairs homeostatic renewal of the intestinal epitheliumCell Death & DiseaseSeptember 28, 2023Anurag Kumar Singh, Amrita Rai, Anja Weber, Martin Gericke, Klaus-Peter Janssen, Markus Moser, Guido Posernantibodies used were cleaved caspase-3 (1:500, #9664, Cell Signaling, Leiden, Netherlands), Ki-67 (1:500, #9129, Cell Signaling) and phospho-histone H3 (Ser10) (1:1000, #06-570, Merck, Darmstadt, DE). Organoids were

(11) Long-term PM2.5 exposure disrupts corneal epithelial homeostasis by impairing limbal stem/progenitor cells in humans and rat modelsParticle and Fibre ToxicologySeptember 27, 2023Shengjie Hao, Zhijian Chen, Yuzhou Gu, Lu Chen, Feiyin Sheng, Yili Xu, Di Wu, Yu Han, Bing Lu, Shuying Chen, et al.(Leica, Wetzla, Germany). The antibodies used in this study were: Ki67 (1:400, Cell Signaling Technology #9129s), K12 (1:200, Santa Cruz Biotechnology #sc-515,882), K10 (1:200, Abcam #ab76318), and CD45 (1:150, Abcam

(12) miR-92a-3p regulates cisplatin-induced cancer cell deathCell Death & DiseaseSeptember 13, 2023Romain Larrue, Sandy Fellah, Nihad Boukrout, Corentin De Sousa, Julie Lemaire, Carolane Leboeuf, Marine Goujon, Michael Perrais, Bernard Mari, Christelle Cauffiez, et al.min and blocked with 3% BSA in TBS for 30 min. Incubation with primary antibody rabbit anti-Ki-67 (#9129, Cell Signaling Technology) was performed in a blocking solution BSA (1%) at 37 °C for 1 h. After three

(13) TP53RK Drives the Progression of Chronic Kidney Disease by Phosphorylating Birc5Advanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2023Wu M, Jin Q, Xu X, Fan J, Chen W, Miao M, Gu R, Zhang S, Guo Y, Huang S, et al.(1:200, Cat #ab469, Abcam), anti‐p‐Birc5 (1:50, Cat #PA1‐16853, Invitrogen), anti‐Ki‐67 (1:200, Cat #9129T, Cell Signaling Technology), anti‐S100A4 (1:50, Cat #A19109, Abclonal or 1:20, Cat #66489‐1‐Ig, ProteinTech Group

(14) Circ_0001946 Promotes the Development of Acute Myeloid Leukemia by Upregulating PDL1Turkish Journal of HematologySeptember 1, 2023Guohui Li, Conghui Zhu, Dan Qiao, Renan Chensubsequently incubated with blocking solution for 30 min and then with primary Ki67 antibody (1:200; Cat. No. 9129, Cell Signaling Technology, USA) overnight in a cold room. The next day, cells were rinsed and incubated

(15) MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongationNucleic acids researchAugust 25, 2023Jin B, Zhu J, Pan T, Yang Y, Liang L, Zhou Y, Zhang T, Teng Y, Wang Z, Wang X, et al.antibodies against menin (A300-105A, Bethyl Laboratories, 1:4000), γH2AX (80312, CST, 1:1000) and Ki67 (9129, CST, 1:400). Negative controls were treated identically except no primary antibody was added. Pictures

(16) METTL3 drives NAFLD-related hepatocellular carcinoma and is a therapeutic target for boosting immunotherapyCell Rep MedAugust 15, 2023Yasi Pan, Huarong Chen, Xiang Zhang, Weixin Liu, Yanqiang Ding, Dan Huang, Jianning Zhai, Wenchao Wei, Jun Wen, Danyu Chen, et al.29441-1-AP; RRID: AB_2918307 Anti-SREBP2,R&D Systems,Cat# AF7119SP Anti-Ki-67,Cell Signaling,Cat# 9129; RRID: AB_2687446 Anti-Phospho-p44/42 MAPK(Erk1/2),Cell Signaling,Cat# 9101L Anti-Phospho-Jak1(Ty1034/1035),Cell Signaling

(17) Loss of SYNCRIP unleashes APOBEC-driven mutagenesis, tumor heterogeneity, and AR-targeted therapy resistance in prostate cancerCancer cellAugust 14, 2023Li X, Wang Y, Deng S, Zhu G, Wang C, Johnson NA, Zhang Z, Tirado CR, Xu Y, Metang LA, et al.Technolgoy,Cat#4874S; RRID: AB_2121214 Rabbit monoclonal anti-Ki 67 (D3B5),Cell Signaling Technology,Cat#9129S; RRID: AB_2687446 Rabbit anti-Phospho Histone H2A.X (Ser139),Cell Signaling Technology,Cat #2577S;

(18) Integrative omics reveals rapidly evolving regulatory sequences driving primate brain evolutionMolecular Biology and EvolutionAugust 3, 2023Xiao-Lin Zhuang, Jin-Jin Zhang, Yong Shao, Yaxin Ye, Chun-Yan Chen, Long Zhou, Zheng-bo Wang, Xin Luo, Bing Su, Yong-Gang Yao, et al.sectioning. The following antibodies were used for immunostaining: primary antibody Ki67 (Cell Signaling, 9129) or BrdU (Abcam, ab92837); secondary Alexa Fluor 555 antibody, A-21428 or A-21437 (Invitrogen); and

(19) A superoxide-driven redox state promotes geroconversion and resistance to senolysis in replication-stress associated senescenceRedox BiologyAugust 1, 2023Le Luo, Shazib Pervaiz, Marie-Veronique ClementLAMP1 (Cat. #ab25630; Abcam). Primary antibodies obtained from Cell Signaling Technology: Ki-67 (Cat. #9129), phospho-histone H2A.X (Ser139) (Cat. #2577), p53 (#9282), FoxM1 (#5436), Phospho-Histone H2A.X (Ser139)

(20) Age-related decline in hippocampal tyrosine phosphatase PTPRO is a mechanistic factor in chemotherapy-related cognitive impairmentJCI InsightJuly 24, 2023Zhimeng Yao, Hongmei Dong, Jianlin Zhu, Liang Du, Yichen Luo, Qing Liu, Shixin Liu, Yusheng Lin, Lu Wang, Shuhong Wang, et al.immunofluorescent staining, sections were incubated with antibodies against the following proteins: Ki67 (1:400; catalog 9129, Cell Signaling Technology), DCX (1:200; catalog sc-217390, Santa Cruz Biotechnology), PSD95 (1:200;

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2/11/2022Ki-67 Antibody With Consistent Results I RIbeiroGood ICC/IF staining in mouse and human cells.

Theranostics

Journal of the American Heart Association

International Journal of Molecular Sciences

Aging (Albany NY)

Redox biology

Ki-67 (D3B5) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Ki-67 (D3B5) Rabbit mAb from Cell Signaling Technology

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